Journal: The Journal of Neuroscience
Article Title: Clustered Fine Compartmentalization of the Mouse Embryonic Cerebellar Cortex and Its Rearrangement into the Postnatal Striped Configuration
doi: 10.1523/JNEUROSCI.1710-12.2012
Figure Lengend Snippet: Antibody specification
Article Snippet: After immunohistochemistry, some sections were fluorescently counterstained with 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI; -cellstain-DAPI, D212, Dojindo). table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Immunogen Manufacturer, species, mono- or polyclonal, catalog and lot no Dilution used Primary antibodies Calbindin-D28k Calbindin-D28k purified from chicken gut was used as the immunogen Sigma-Aldrich, mouse monoclonal, Cat. # C-8666, Lot # 058H4812 1:6000 Calbindin-D28k Synthetic peptide derived from amino acids 185-199 of human Calbindin-D-28K AnaSpec, rabbit polyclonal, Cat. # 53283, Lot # GL141 1:4000 EphA4 Purified, NS0-derived, recombinant mouse EphA4 extracellular domain R&D Systems, goat polyclonal, Cat. # AF641, Lot # BVX0308091 1:1000 FoxP2 Synthetic peptide derived from C-terminal amino acids of isoforms I, II, IV, and V of human FOXP2: REIEEEPLSEDLE Everest Biotech, goat polyclonal, Cat. # EB05226, Lot # 160409 1:5000 for fluorescent, 1:10000 for DAB FoxP2 A KLH-conjugated synthetic peptide between 664 and 693 amino acids from the C-terminal region of human FOXP2 Abgent, rabbit polyclonal, Cat. # AP5753b, Lot # SA100916AA 1:1000 Pcdh10 The cytoplasmic domain of the OL-pc isoform ( Aoki et al., 2003 ) Millipore, rat monoclonal, clone 5G10, Cat. # MABT20, Lot # NRG1759424 1:1600 PLCβ4 Amino acids 876-1115 of PLCβ4 of human origin Santa Cruz Biotechnology, rabbit polyclonal, Cat. #sc-20760, Lot # A1508 1:150 RORα Amino acids 196-260 mapping within an internal region of RORα2 of human origin Santa Cruz Biotechnology, rabbit polyclonal, Cat. # sc-28612, Lot # I0909 1:100 Secondary antibodies Anti-goat IgG, biotin-conjugated Vector Laboratories, rabbit, Cat. # BA-5000 1:333 Anti-rabbit IgG, biotin-conjugated Vector Laboratories, goat, Cat. # BA-1000 1:333 Anti-mouse IgG, biotin-conjugated Vector Laboratories, goat, Cat. # BA-9200 1:333 Anti-goat IgG, fluorescein-conjugated Jackson ImmunoResearch, donkey, Cat. # 705-095-147 1:333 Anti-goat IgG, DyLight 488-conjugated Jackson ImmunoResearch, donkey, Cat. # 705-485-147 1:400 Anti-rabbit IgG, Texas Red-conjugated Jackson ImmunoResearch, donkey, Cat. # 711-075-152 1:333 Anti-rat IgG, DyLight 594-conjugated Jackson ImmunoResearch, donkey, Cat. # 712-515-153 1:333 Open in a separate window The data are based on manufacturer's data sheets and in some cases on communications with manufacturers.
Techniques: Purification, Derivative Assay, Recombinant, Plasmid Preparation
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